Journal: Translational Cancer Research

Article Title: MicroRNA-497-5p negatively regulates the proliferation and cisplatin resistance of non-small cell lung cancer cells by targeting YAP1 and TEAD1

doi: 10.21037/tcr.2019.10.03

Figure Lengend Snippet: Primer sequences for quantitative real-time PCR

Article Snippet: YAP1 and TEAD1 expression pcDNA3.1 plasmids were purchased from Origene (Beijing, China).

Techniques: Sequencing

Journal: Translational Cancer Research

Article Title: MicroRNA-497-5p negatively regulates the proliferation and cisplatin resistance of non-small cell lung cancer cells by targeting YAP1 and TEAD1

doi: 10.21037/tcr.2019.10.03

Figure Lengend Snippet: YAP1 and TEAD1 are targeted by miR-497-5p. (A) Sequence comparison between miR-497-5p and the 3’-UTR region of YAP1 and TEAD1. A549 cells were transfected with miR-497-5p mimic or miR-497-5p inhibitor, the levels of YAP1 and TEAD1 are detected by qRT-PCR (B) and Western blot (C). (D) A549 and H1299 cells were co-transfected with miR-497-5p mimic and luciferase vectors carrying 3’-UTR of YAP1 or TEAD1, the luciferase activity was measured. (E) The levels of YAP1 and TEAD1 in clinical cancer specimens were evaluated by qRT-PCR. (F) The correlation between miR-497-5p and YAP1/TEAD1 was evaluated by Spearman’s rank correlation test. Data were mean ± SD and were representative of three independent experiments. *P<0.05; **P<0.01; ***P<0.001.

Article Snippet: YAP1 and TEAD1 expression pcDNA3.1 plasmids were purchased from Origene (Beijing, China).

Techniques: Sequencing, Comparison, Transfection, Quantitative RT-PCR, Western Blot, Luciferase, Activity Assay

Journal: Translational Cancer Research

Article Title: MicroRNA-497-5p negatively regulates the proliferation and cisplatin resistance of non-small cell lung cancer cells by targeting YAP1 and TEAD1

doi: 10.21037/tcr.2019.10.03

Figure Lengend Snippet: MiR-497-5p regulates the function of lung cancer cells via suppressing YAP1 and TEAD1. A549 and H1299 cells were co-transfected with miR-497-5p mimic and YAP1- or TEAD1-expressing plasmids, cell proliferation was measured by CCK-8 assay (A) and colony-formation assay (B). Cell cycle (C) and cell apoptosis (D) were evaluated by flow cytometry. The expression of YAP1 or TEAD1 was determined by qRT-PCR (E) and Western blot (F). Data were mean ± SD and were representative of three independent experiments. *P<0.05; **P<0.01.

Article Snippet: YAP1 and TEAD1 expression pcDNA3.1 plasmids were purchased from Origene (Beijing, China).

Techniques: Transfection, Expressing, CCK-8 Assay, Colony Assay, Flow Cytometry, Quantitative RT-PCR, Western Blot

Journal: Translational Cancer Research

Article Title: MicroRNA-497-5p negatively regulates the proliferation and cisplatin resistance of non-small cell lung cancer cells by targeting YAP1 and TEAD1

doi: 10.21037/tcr.2019.10.03

Figure Lengend Snippet: MiR-497-5p sensitizes lung cancer cells to cisplatin through the inhibition of YAP1 and TEAD1. (A) The expression miR-497-5p, YAP1 and TEAD1 in A549 cells and A549/DDP cells was evaluated by qRT-PCR. (B) The expression YAP1 and TEAD1 in A549 cells and A549/DDP cells was evaluated by western blot. (C,D) A549/DDP cells was transfected with miR-497-5p mimic followed by treatment with cisplatin, cell viability was evaluated by CCK-8 assay (C), cell apoptosis was measured by flow cytometry (D). (E) The levels of YAP1 and TEAD1 was evaluated in cisplatin-challenged A549/DDP cells by western blot. Data were mean ± SD and were representative of three independent experiments. *P<0.05; **P<0.01.

Article Snippet: YAP1 and TEAD1 expression pcDNA3.1 plasmids were purchased from Origene (Beijing, China).

Techniques: Inhibition, Expressing, Quantitative RT-PCR, Western Blot, Transfection, CCK-8 Assay, Flow Cytometry